]> Mapping 1 Channel number 1

High Frequency

Mapping 2 Channel number 2

Low Frequency

Correction 3 Background Well Type Background correction method Local correction value Median High Frequency

Corrected High Frequency

Correction 4 Background Well Type Background correction method Local correction value Median Low Frequency

Corrected Low Frequency

Ratio 5 Ratio Denominator Corrected High Frequency Ratio Numerator Corrected Low Frequency

Ratio

Activity 6 Assay mode Antagonist Mode High Control Well Type Background Low Control Well Type Compound Low Frequency

Percent Inhibition

Hit Selection Hit Selection Channel High Frequency Hit Selection Method Std Deviation Hit Selection Threshold -9 Validation Validation Channel Percent Stimulation Validation Method Absolute Cut Off Validation Threshold 500 Validation Validation Channel Percent Inhibition Validation Method Std Deviation Validation Threshold 33 Validation Validation Channel Corrected High Frequency Validation Method Std Deviation Validation Threshold 432 Validation Validation Channel Low Frequency Validation Method Absolute Cut Off Validation Threshold 66 Validation Validation Channel High Frequency Validation Method Std Deviation Validation Threshold -98