Analysis of the Kluyveromyces lactis Heat Shock Factor:
A Representative of the Helix-Turn-Helix
Transcription Factor Family





SUMMARY

DISEASE

FAMILY

FUNCTION

STRUCTURE

PROPERTIES

SEQUENCE-FUNCTION

STRUCTURE-FUNCTION

EVOLUTION

STRUCTURE COMPARISON



Properties



Theoretical Physico-Chemical Properties of the Kluyveromyces lactis Heat Shock Factor


Number of amino acids - 677

Molecular mass - 7542008

Isoelectric point - 5.55



Composition of amino acids

     Ala  (A)    25     3,69 %
     Arg  (R)    27     3,99 %
     Asn  (N)    65     9,60 %
     Asp  (D)    37     5,47 %
     Cys  (C)     0     0,00 %
     Gln  (Q)    61     9,01 %
     Glu  (E)    41     6,06 %
     Gly  (G)    50     7,39 %
     His  (H)    22     3,25 %
     Ile  (I)    30     4,43 %
     Leu  (L)    55     8,12 %
     Lys  (K)    33     4,87 %
     Met  (M)    16     2,36 %
     Phe  (F)    19     2,81 %
     Pro  (P)    44     6,50 %
     Ser  (S)    72    10,64 %
     Thr  (T)    27     3,99 %
     Trp  (W)     6     0,89 %
     Tyr  (Y)    15     2,22 %
     Val  (V)    32     4,73 %

     Asx  (B)     0     0,00 %
     Glx  (Z)     0     0,00 %


Predicted Peptide Fragments

The selected enzyme is: Trypsin

Maximum number of missed cleavages (MC): 0

All cysteines in reduced form.

Methionines have not been oxidized.

Displaying peptides with a mass bigger than 500 Dalton.

Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H]+.

      mass position #MC peptide sequence
      9831.639
      477-566
      0
      		 IVGQSPPPQQQQQQQQQQGQ PQGQQFSYPIQGGNQMMNQL GSPIGTQVGSPVGSQYGNQY GNQYSNQFGNQLQQQTSRPA LHHGSNGEIR
      7055.477
      53-121
      0
      		 DDSNVDEPLIEDIVNPSLDP EGVVSAEPSNEVGTPLLQQP ISLDHVITRPASAGGVYSIG NSSTSSAAK
      6599.970
      381-440
      0
      		 TIGNGFQPDLIHELSDMQVN HMSNNNHNNTGNINPNAYHN ETDDPMANVFGPLTPTDQGK
      5494.536
      1-52
      0
      		 MGHNDSVETMDEISNPNNIL LPHDGTGLDATGISGSQEPY GMVDVLNPDSLK
      5012.244
      607-653
      0
      		 LNPGPGEDGNTPIFPELNMP SYFANTGGSGQSEQPSDYGD SQIEELR
      3785.713
      122-157
      0
      		 LSDGDLTNATDPLLNNAHGH GQPSSESQSHSNGYHK
      2908.526
      299-325
      0
      		 SNTNILGGTTNAEVDIHILL NELETVK
      2877.379
      567-592
      0
      		 ELTPSIVSSDSPDPAFFQDL QNNIDK
      1828.934
      213-228
      0
      		 FIHWSTSGESIVVPNR
      1746.854
      593-606
      0
      		 QEESIQEIQDWITK
      1440.780
      158-170
      0
      		 QGQSQQPLLSLNK
      1384.612
      458-470
      0
      		 SMSSSSSSNLNQR
      1277.674
      326-336
      0
      		 YNQLAIAEDLK
      1253.652
      369-380
      0
      		 FLSSVFGPNSAK
      1242.543
      276-284
      0
      		 WEFENENFK
      1224.633
      356-365
      0
      		 HQSQQQVLEK
      1176.562
      251-259
      0
      		 QLNMYGWHK
      1167.506
      265-275
      0
      		 SGSMLSNNDSR
      1148.631
      288-296
      0
      		 EYLLENIVR
      1064.527
      242-250
      0
      		 HSNFASFVR
      992.487
      201-208
      0
      		 LWSMVNDK
      959.556
      231-238
      0
      		 FVQEVLPK
      935.429
      341-347
      0
      		 DNEMLWK
      902.521
      193-200
      0
      		 ARPAFVNK
      862.467
      441-447
      0
      		 VPLQDYK
      766.384
      657-662
      0
      		 LHEPDR
      751.323
      348-353
      0
      		 ENMMAR
      729.364
      471-476
      0
      		 QSPQNR
      639.298
      663-667
      0
      		 SFEEK
      588.335
      260-264
      0
      		 VQDVK
      577.330
      188-192
      0
      		 LSTTR
      569.304
      177-181
      0
      		 AHVDK
      560.279
      668-672
      0
      		 NNGQK
      541.357
      448-451
      0
      		 LRPR
      508.263
      182-185
      0
      		 HHSK