Gel Filtration Chromatography / Native-Denaturing PAGE
Molecular Mass : 12,394 Daltons (+/- 500 Daltons)
Chromatofocusing / Isoeletic Focusing
Isoelectric Point : 9.89 (+/- 0.1)
Complete Proteolytic/Chemical Digestion
Total no. amino acids : 114
Amino acid composition (absolute, relative):
Ala (A) 15 13,16 %
Arg (R) 3 2,63 %
Asn (N) 6 5,26 %
Asp (D) 4 3,51 %
Cys (C) 2 1,75 %
Gln (Q) 4 3,51 %
Glu (E) 7 6,14 %
Gly (G) 11 9,65 %
His (H) 2 1,75 %
Ile (I) 3 2,63 %
Leu (L) 8 7,02 %
Lys (K) 14 12,28 %
Met (M) 2 1,75 %
Phe (F) 4 3,51 %
Pro (P) 8 7,02 %
Ser (S) 5 4,39 %
Thr (T) 6 5,26 %
Trp (W) 1 0,88 %
Tyr (Y) 6 5,26 %
Val (V) 3 2,63 %
Peptide Mapping
Experimental parameters:
The selected enzyme is: Trypsin
Maximum number of missed cleavages (MC): 0
All cysteines in reduced form.
Methionines have not been oxidized.
Displaying peptides with a mass bigger than 500 Dalton.
Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H]+.
The peptide masses for your protein are:
1505.69
1411.62
1334.61
1209.65
1172.62
906.53
890.43
791.45
678.38
643.35
632.33