Gel Filtration Chromatography / Native-Denaturing PAGE
Molecular mass : 33,643 Daltons (+/- 1,500 Daltons)
Chromatofocusing /Isoelectric Focusing
Isoelectric Point : 4.71 (+/- 0.2)
Complete Proteolytic/Chemical Digestion
Total no. amino acids : 312
Amino acid composition (absolute, relative):
Ala (A) 24 7,69 %
Arg (R) 14 4,49 %
Asn (N) 11 3,53 %
Asp (D) 17 5,45 %
Cys (C) 0 0,00 %
Gln (Q) 8 2,56 %
Glu (E) 24 7,69 %
Gly (G) 31 9,94 %
His (H) 1 0,32 %
Ile (I) 23 7,37 %
Leu (L) 27 8,65 %
Lys (K) 18 5,77 %
Met (M) 8 2,56 %
Phe (F) 7 2,24 %
Pro (P) 13 4,17 %
Ser (S) 18 5,77 %
Thr (T) 22 7,05 %
Trp (W) 0 0,00 %
Tyr (Y) 13 4,17 %
Val (V) 33 10,58 %
Peptide Mapping
Experimental parameters:
The selected enzyme is: Trypsin
Maximum number of missed cleavages (MC): 0
All cysteines in reduced form.
Methionines have not been oxidized.
Displaying peptides with a mass bigger than 500 Dalton.
Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H]+.
The peptide masses (Daltons)for your protein are:
4759.43 3108.59 2559.31 2424.23 1993.09 1984.00 1952.09 1691.83 1621.88 1349.73 1215.67 1020.48 815.46 675.36 664.33 648.36 578.27 573.34