Gel Filtartion Chromatography / Naive-Denaturing PAGE
Molecular Mass : 39,189 Daltons (+/- 1,200 Daltons)
Chroamtofocusting /Isoelectric Focusing
Isoelectric Point: 4.41 (+/- 0.25)
Complete Proetolytic/Chemical Digestion
Total no. amino acids : 339
Amino acid composition (absolute, relative):
Ala (A) 19 5,60 %
Arg (R) 7 2,06 %
Asn (N) 27 7,96 %
Asp (D) 27 7,96 %
Cys (C) 3 0,88 %
Gln (Q) 12 3,54 %
Glu (E) 18 5,31 %
Gly (G) 28 8,26 %
His (H) 3 0,88 %
Ile (I) 15 4,42 %
Leu (L) 19 5,60 %
Lys (K) 22 6,49 %
Met (M) 12 3,54 %
Phe (F) 18 5,31 %
Pro (P) 13 3,83 %
Ser (S) 21 6,19 %
Thr (T) 18 5,31 %
Trp (W) 15 4,42 %
Tyr (Y) 25 7,37 %
Val (V) 17 5,01 %
Peptide Maping
Experimental parameters
The selected enzyme is: Trypsin
Maximum number of missed cleavages (MC): 0
All cysteines in reduced form.
Methionines have not been oxidized.
Displaying peptides with a mass bigger than 500 Dalton.
Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H]+.
The peptide masses for your protein are:
7192.37
3396.50
3295.62
3272.57
2543.14
2191.98
1665.83
1642.82
1457.65
1390.59
1334.64
1199.58
1138.56
1050.49
1040.48
914.48
772.40
751.37
663.35
505.23