Gel Filtration Chromatography / Native-Denaturing PAGE
Molecular mass : 79,140 Daltons (+/- 2,000 Daltons)
Chromatofocusing
Isoelectric Point : 6.08 (+/- 0.25)
Total no. amino acids : 713
Amino acid composition (absolute, relative):
Ala (A) 56 7,85 %
Arg (R) 30 4,21 %
Asn (N) 23 3,23 %
Asp (D) 48 6,73 %
Cys (C) 7 0,98 %
Gln (Q) 22 3,09 %
Glu (E) 38 5,33 %
Gly (G) 50 7,01 %
His (H) 20 2,81 %
Ile (I) 44 6,17 %
Leu (L) 58 8,13 %
Lys (K) 47 6,59 %
Met (M) 8 1,12 %
Phe (F) 26 3,65 %
Pro (P) 40 5,61 %
Ser (S) 51 7,15 %
Thr (T) 47 6,59 %
Trp (W) 12 1,68 %
Tyr (Y) 31 4,35 %
Val (V) 55 7,71 %
Peptide Mapping
Experimental parameters:
The selected enzyme is: Trypsin
Maximum number of missed cleavages (MC): 0
All cysteines in reduced form.
Methionines have not been oxidized.
Displaying peptides with a mass bigger than 500 Dalton.
Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H]+.
The peptide masses (daltons) for your protein are:
6907.45 5752.71 4102.13 2988.36 2922.38 2460.32 2423.21 2389.10 2267.14 2253.05 2241.17 1987.97 1888.01 1861.99 1695.77 1581.81 1371.65 1365.75 1333.64 1310.66 1218.58 1196.60 1180.68 1177.56 1150.55 1138.71 1133.58 1092.58 1021.50 977.46 915.49 905.46 888.43 871.52 815.47 811.47 801.45 786.46 748.35 658.35 589.36 585.30 547.32 541.25