Gel Filtration Chromatography / Native-Denaturing PAGE
Molecular mass: 81,042 Daltons (+/- 4,000 Daltons)
Chromatofocusing /Isoelectric Focusing
Isoelectric point: 5.67 (+/-0.25)
Complete Proteolytic/Chemical Digestion
Total no. amino acids: 730
Amino acid composition (absolute, relative):
Ala (A) 60 8,22 %
Arg (R) 35 4,79 %
Asn (N) 39 5,34 %
Asp (D) 29 3,97 %
Cys (C) 9 1,23 %
Gln (Q) 27 3,70 %
Glu (E) 41 5,62 %
Gly (G) 52 7,12 %
His (H) 13 1,78 %
Ile (I) 36 4,93 %
Leu (L) 55 7,53 %
Lys (K) 25 3,42 %
Met (M) 22 3,01 %
Phe (F) 35 4,79 %
Pro (P) 37 5,07 %
Ser (S) 73 10,00 %
Thr (T) 54 7,40 %
Trp (W) 17 2,33 %
Tyr (Y) 30 4,11 %
Val (V) 41 5,62 %
Peptide Mapping
Experimental Parameters:
The selected enzyme is: Trypsin
Maximum number of missed cleavages (MC): 0
All cysteines in reduced form.
Methionines have not been oxidized.
Displaying peptides with a mass bigger than 500 Dalton.
Using monoisotopic masses of the occurring amino acid residues and giving peptide masses as [M+H]+.
The peptide fragment masses (daltons) for this protein are:
6272.07 3884.73 3883.93 3876.84 3861.82 3624.83 3168.51 2828.30 2649.21 2473.12 2340.07 2163.98 2153.14 2110.88 1772.94 1747.79 1744.89 1725.90 1697.88 1616.78 1614.75 1591.72 1541.73 1398.71 1349.68 1272.57 1245.79 1199.57 1170.68 1151.61 1016.52 943.42 888.44 743.44 730.42 675.34 616.37 503.28